Phenotypic Identification and Genotypic Characterization of Plasmid-Mediated AmpC ?-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Isolates in Iran

نویسندگان

چکیده

One of the mechanisms Klebsiella pneumoniae and Escherichia coli resistance to ?-lactam antibiotics is production ?-lactamase enzymes. Among these are AmpC ?-lactamases, which confer a class antibiotics. However, little known about ?-lactamases K. E. clinical isolates in Qazvin, Iran. This study was designed assess ??lactamases-producing strains also identify prevalence ??lactamases genes. Antimicrobial susceptibility tests were performed on 435 using disk diffusion technique. Plasmid-mediated genes studied multiplex PCR assay. The ?-lactamase-producer by employing cefoxitin test, induction cefoxitin-EDTA boronic acid test. Our results showed that 46 (18.4%) cefoxitin-insensitive isolates, 10 (21.7%) positive for genes, among them 4 (8.69%) blaDHA 6 (13%) blaCIT Of 57 (30.4%) (17.5%) gene with (6.34%) (9.5%) respectively. no MOX, ACC, FOX, or EBC detected isolates. Considering different confirmatory phenotypic tests, test higher discriminatory power detecting ?-lactamase-producing strains. specificity sensitivity 77%, 100% pneumonia 70%, 90% than other two Also, authors demonstrated high rate certain antibiotics, such as cefuroxime, trimethoprim-sulfamethoxazole, ampicillin, cefotaxime. In conclusion, our provided valuable information regarding plasmid-mediated content, antibiotic resistance, from sources.

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ژورنال

عنوان ژورنال: Current Microbiology

سال: 2021

ISSN: ['0343-8651', '1432-0991']

DOI: https://doi.org/10.1007/s00284-021-02479-9